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Topical protocols deliver the peptide directly to dermal tissue at the application site, achieving high local concentrations without systemic distribution

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AMPK activation is an energy sensorit works most effectively when cellular ATP is low, which occurs during fasting, caloric restriction, or intense training

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Lower NAD+ means reduced sirtuin activity (SIRT1, SIRT3), which further impairs mitochondrial function and fatty acid oxidation in fat cells