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The disease is primarily caused by the loss of dopamine-producing neurons in the substantia nigra, a part of the brain that regulates movement

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They are widely used today to help rebalance the gut microbiota

Cells were extensively washed with PBS, fixed in 4% PFA for 15 min, permeabilized and blocked with 0.01% saponin/10% normal goat/PBS serum and stained with the primary antibodies in the same blocking solution in a humid chamber