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Samples were resuspended in 2% acetonitrile (ACN), 0.05%TFA and peptide concentrations were estimated with 205 nm absorbance readings (Nanodrop, Thermo Fischer)

Before administration, the bacterial suspension was adjusted to a final concentration of 1 10 9 CFU/mL with sterile MRS medium, and freshly prepared immediately before feeding to piglets to ensure the viable bacterial count

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However, serum alkaline aminotransferase, total cholesterol triglycerides, albumin, and high density remained unchanged in all animals following therapeutic and supra-therapeutic administrations

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