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Mass spectrometric analyses of eluted peptide lysates were performed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific) coupled with a TriVersa NanoMate (Advion, Ltd., Harlow, UK)

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The next points to be marked are the midpoint of the columellar base (SN), the highest points of the philtral columns (CPHSR, CPHSL) and the commissures (CHR, CHL)

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In addition to either the semaglutide or placebo, participants received lifestyle intervention except for those who were part of the STEP 3 group