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Clinically, multiple cohort studies support a causal link between vitamin D deficiency and AKI risk
Immunoblotting and antibodies Whole-cell extracts were prepared by resuspension of cell pellets in lysis buffer (67.5 mM Tris pH 6.8, 25 mM NaCl, 0.5 mM EDTA, 12.5% glycerol, 0.25% SDS and 1 mM DTT)
Methods Simplified human intestinal microbiota SIHUMIx The extended simplified human gut microbiota (SIHUMIx) comprises of eight species including Anaerostipes caccae (DSMZ 14662), Bacteroides thetaiotaomicron (DSMZ 2079), Bifidobacterium longum (NCC 2705), Blautia producta (DSMZ 2950), Clostridium butyricum (DSMZ 10702), Clostridium ramosum (DSMZ 1402), Escherichia coli K-12 (MG1655) and Lactobacillus plantarum (DSMZ 20174) ( in vitro culture ( Experimental set-up Continuous flow cultivation of SIHUMIx was carried out in an in vitro bioreactor system (Multifors 2 chemostat, Infors, Switzerland)
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