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Blood samples were collected from the jugular vein of each horse

Levels of PPAT, the key enzyme of de novo purine nucleotide synthesis, are increased in TC tissues, enhancing PKM2 expression and activating extracellular signal-regulated kinase (ERK) and signal transducer of activation 3 (STAT3) signaling pathways [73]

Such enzymatic reaction generates a reactive intermediate known as N-acetyl-p-benzoquinoneimine (NAPQI) (40) (Figure 2)

Not to get too technical, but vitamin C is chemically a pro-oxidant

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However, due to the limited range of administered dosages of NAC, subgroup analysis based on the dosage was not performed on LH, FSH and TT levels