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1.18 Diagnosis, clinical presentation and treatment The diagnosis of Hashimotos thyroiditis (HT) is made based on the clinical symptoms and biochemical findings of hypothyroidism

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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

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Warnings Once opened, store in a cool (under 25C), dry and dark place

Cell Cycle 7 , 27692773 (2008)