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Before the experiment, the cells were loaded with 0.2% Fluo-4 AM, which was dissolved in HEPES-buffered Hanks Balanced Salt Solution (HBSS, Thermofisher) containing 1 mM CaCl2, 1 mM MgCl2, and 0.5% probenecid (Thermofisher) for one hour

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However, if you have a sensitive gut, an overload of glutathione can cause a slight disruption to digestion, which is why experts suggest starting with a smaller dose and working your way up

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