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doi: 10.1111/1750-3841.13711 30

For enzyme kinetic assays, the reactions contained 50 mM Tris-HCl (pH 8.0), 250 mM NaCl, FSP1 proteins at a constant final concentration (50 nM cFSP1, 25 nM hFSP1, 25 nM 6-hydroxy-FAD-reconstituted hFSP1 or 50 nM FAD-reconstituted hFSP1 in aerobic condition, or 10 nM hFSP1 in anaerobic condition), either with 400 M CoQ 1 and varied NAD(P)H concentration (to determine kinetics for NAD(P)H) or with 500 M NAD(P)H and varied CoQ 1 concentration (to determine kinetics for NAD(P)H)

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