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Quantitative real-time PCR(RT-PCR) was performed on a ViiATM 7 RT-PCR system (Applied Biosystems Inc.) with SYBR-green ChamQTM SYBR Color qPCR Master Mix (Vazyme)

Thalidomide has the anti-inflammatory properties via selective inhibitory activity of tumour necrosis factor- (TNF) and the anti-angiogenesis effect via downregulating VEGF levels

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