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In contrast, sleep-deprived mice treated with saline (SD) had a significant decrease in the learning curve slope indicating learning impairment without GHK

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After they were rinsed with phosphate-buffered saline, they were incubated with a rodent monoclonal secondary antibody (Cat# K3468, EnVision+ System Horseradish Peroxidase Labeled Polymer

Schielke et al., 2010), which, as mentioned above, was implicated in diverse functions including adherence to epithelial cells, intracellular survival, vacuolar escape, interaction with dynein, and modulation of apoptosis/pyroptosis (Schmitt et al., 2007