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Laboratory studies use these receptor systems to investigate peptide mediated signaling pathways involved in neuroendocrine communication, nutrient sensing, and metabolic regulation within experimental models

GHSR exhibits significant expression within the central nervous system

For further details of the protocol please see Katre et al

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Based on such metabolic formal screening, none of the five subjects were found to have clinical or biochemical evidence of an inborn error of metabolism including possible mitochondrial disorders

For silencing FOXO4, specific small interfering RNA (siRNA) was employed, with a negative control siRNA, and transfection was conducted using Lipofectamine 3000 [2]