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The IC 50 values of EA and PRT 4165 when used alone are 13 M and 59 M, respectively, and when used together achieve an IC 50 value at concentrations of 2.9 M for EA and 13.1 M for PRT 4165
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This was performed by high-pressure liquid chromatography using a LC-20A Shimadzu equipment (Shimadzu GmbH, Duisburg) with an interphase module (CBM-20A, Shimadzu GmbH) and electrochemical detection (ESA Biosciences, Inc., Chelmsford, MA), with the upstream electrode set at +100 mV (for compounds with low oxidation potential) and downstream electrode set between +100 and +650 mV (in +50 mV increments) for each injected standard to determine the optimum potential for detection
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