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These were cloned into the pET28a(+) expression vector (Novagen) using the NdeI and BamHI restriction sites and sequence verified

PMID: 7987362

It is often called a "twincretin" because it mimics both GLP-1 and glucose-dependent insulinotropic polypeptide (GIP)

Acta Biochim Biophys Sin (Shanghai) 42(3):183194 Skalny AA, Tinkov AA, Medvedeva YS, Alchinova IB, Karganov MY, Skalny AV, Nikonorov AA (2015) Effect of short-term zinc supplementation on zinc and selenium tissue distribution and serum antioxidant enzymes

It has been used for centuries for its flavor and potential health benefits

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