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Ethics statement The animal study was reviewed and approved by the Laboratory Animal Welfare and Ethics Committee of Fourth Military Medical University

For people born in 2025, the projected average age for pregnancy is 36 (currently 30.9 years)

one was a control, and the other was labeled as the DSIP group

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR