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In general, vitamin B12 overdose probably isn't something you need to worry about unless you're taking extremely high dosages of supplements containing this vitamin

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J., & Bernal, G

Using wrong water types or mixing too vigorously can compromise peptide bonds

Hygiene and Cleanliness: If you're using a multi-dose B12 vial, always use a new, sterile needle and syringe for each injection to prevent contamination and maintain sterility