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Furthermore, it conjugates with toxins in the liver via Glutathione S-transferase (GST) , facilitating their excretion [4]

Measurement of intracellular iron, GSH and MDA For the determination of iron levels, the collected cells and rat prostate tissue (10 mg) were homogenized in pre-cooled iron analysis buffer (Beyotime Biotechnology Co., Ltd

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This may subsequently induce alterations in local gut microbial structure and metabolic products, thereby establishing and exacerbating a vicious cycle of ferroptosis