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The membrane was then incubated with the anti-IRE1 alpha (Inositol requiring enzyme-1 alpha) (p Ser724) antibody (dilution 1:1000

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For the targeted sulfur metabolite quantification approach, the previously established LC-MS conditions were applied 99 with selected reaction monitoring (MRM) using an Ultimate 3000 UPLC system coupled to a Thermo Finnigan TSQ Quantum equipped with HESI (Thermo Fisher Scientific, Waltham, MA)

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