Identification of 38 novel loci for systemic lupus erythematosus and genetic heterogeneity between ancestral groups
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J Urol 1949
Co-expression cell pellets were resuspended in 3 mL assay buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol) and this was then divided into 1.5 mL for each of the different affinity pull-downs
