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MRC/BHF Heart Protection Study of antioxidant vitamin supplementation in 20,536 high-risk individuals: a randomised placebo-controlled trial

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doi:10.1093/jn/134.3.489 Semenova NV, Madaeva IM, Brichagina AS, Kolesnikov SI, Kolesnikova LI

Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)

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