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Vitamin B6: This vitamin is crucial for the proper function of DAO and histamine N-methyltransferase (HNMT), another enzyme involved in histamine metabolism

GSH synthesis occurs in two steps, catalyzed by the enzymes glutamate cysteine ligase and GSS, to form the tripeptide glutathione

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Genet 33 , 6165 (2003)

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Youll use reduced glutathione in your elution buffer to remove your GST-fusion protein from the glutathione agarose beads