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The FDA also recommends examining hard clinical endpoints such hepatic decompensations, transplant free survival, and all-cause mortality, although these results are not necessarily needed prior to drug approval given the long length of follow up potentially required

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later by L-NNA (20 mg/kg i.p.) without or with following administration of PTX (10 mg/kg i.p.), in animals subsequently exposed to 3.5 h of WRS

Control experiments were also performed to confirm that compound 102 would not interfere with the dual-luciferase reporter assay system ( Supplementary Figure S10 )