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The membranes were blocked using Bullet Blocking One for Western Blotting (Nacalai Tesque), incubated overnight at 4C with the primary antibody, and then incubated with an HRP-conjugated secondary antibody

Ou et al., 2019)

The column was washed with 6 bed volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl and 100 mM imidazole, pH 7.4) and the target protein was eluted with 6 bed volumes of elution buffer (50 mM sodium phosphate, 300 mM NaCl and 300 mM imidazole, pH 7.4)

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