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In addition, SHMT2 depletion has been shown to induce mitochondrial respiratory chain deficiency by reducing basal respiration and respiratory capacity, decreasing NAD + /NADH ratios, and lowering the expression levels of respiratory complex I, IV, and V proteins [93]

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The results are calculated by evaluating the increase in OD per minute (slope) for unknowns against the slope for a Standard with known G-6-PD activity