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It was found that the stiffening of the ECM impaired the differentiation of alveolar type II (AT2) epithelial cells into type I (AT1) cells 13,14 , which damaged the self-repairing abilities of alveolar structure

At specific time intervals, 2 mL aliquots were withdrawn from the release medium, followed by addition of an equal volume of fresh medium

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injection at a dose (150 g/200 l