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To induce acute liver injury, 8-week-old NSI mice received two consecutive intraperitoneal injections of DMN (7 mg/kg, Sigma, 1.0% dissolved in saline)

doi: 10.1016/j.ijmm.2018.09.003

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Perhaps most significantly, epigenetic modifications including DNA methylation alterations, histone modifications, and non-coding RNA dysregulation create molecular signatures that contribute to transgenerational inheritance of reproductive dysfunction

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