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The cells were treated as described above for 24 h and then stained with Liperfluo (1 mM) for 30 min at 37 C

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10.3390/ijms24119352 79 Gonzlez-GuardiaL.Yubero-SerranoE

The replacement of the media is essential for removing the CuCl 2 and Cu(OAc) 2 interference before assaying the LDH levels as they are shown to reduce the fluorescence signal read-out within treated wells