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IGF-1 LR3 binds the alpha subunit of the IGF-1 receptor tyrosine kinase, inducing autophosphorylation of the beta subunit on specific tyrosine residues (Y1131, Y1135, Y1136) that create docking sites for insulin receptor substrate (IRS) proteins and Shc adaptor proteins

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To do this, you'll first pull back the plunger to the 1ml mark to draw in air, then inject that air into the BAC water vial

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