Extracted proteins were separated on 812% SDS-PAGE gels and transferred onto PVDF membranes (Millipore, ISEQ00010)
Autoimmunity 47(8):494504 Laudadio I, Fulci V, Palone F, Stronati L, Cucchiara S, Carissimi C (2018) Quantitative assessment of shotgun metagenomics and 16S rDNA amplicon sequencing in the study of human gut microbiome
When cells undergo oxidative stress, the redox homeostasis of the ER is disrupted, which disturbs the function of the ER and finally leads to ER stress
Prolactin signalling occurs via a unique transmembrane prolactin receptor (PRL-R)
This syringe only holds three milliliters of water so were going to have to go back for a second time
Therefore, to identify the role of NF-B and the regulative mechanism between GPX1 and NF-B in SGNs damage induced by peroxynitrite, we measured the NF-B pathway in SGNs after peroxynitrite administration with GPX1 upregulation or deficiency (Figure 8A)