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PPAR is a major transcriptional regulator of fatty acid oxidation and extended PPAR activation causes HCC in rodent mice by mechanisms that involve perturbation of the cell cycle and production of ROS [reviewed by Michalik et al
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For example, low intracellular ATP or high AMP levels can activate AMPK, suppressing mTORC1 and halting biosynthetic processes
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PMC3385825 DailyMed
Thats how glutathione agarose is used to facilitate the purification of GST-fusion proteins and to investigate molecular interactions
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