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3.5 Molecular dynamics (MD) simulation of SR18 complexed to loop remodeled GLP-1R The SR18GLP-1R complex embedded in a POPC-CHL (75:25) bilayer was subjected to MD simulation studies over 1 s at 300 K, similar to the GLP-1GLP-1R complex
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54 In addition, elevated plasma nonesterified fatty acid (NEFA) levels impaired the insulin-induced increase in IRS-1-associated PI3K activity, but no defect in Akt phosphorylation was observed
Materials and methods CRISPR/Cas9 all-in-one plasmid construction and generation of GLA-KO human iPSCs All-in-one CRISPR/Cas9 carrying GFP and gRNA was purchased from Life Technologies in California, USA (Cat
