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For all ECM types tested, the highest values for melanin production were observed at high stiffness (Figs

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Western blotting analysis Total cells lysates were prepared in 2 sample loading buffer (that is, 250 mM Tris-HCl pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 2% -mercaptoethanol, 50 mM sodium fluoride and 5 mM sodium orthovanadate)