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Tirzepatide is supplied in single-dose prefilled pens with a hidden needle designed for subcutaneous injection

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The sections were cleaned in PBS, stained with 3,3-Diaminobenzidine (DAB) solution, dehydrated in graded ethanol solutions (80, 90 and 100%), fixed in xylene, cover-slipped with a mounting medium and allowed to dry by air

All other TBS proteins analyzed were expressed at similar levels in all transgenic lines ( Supplementary Figure 3D )

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