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Chang, Y.-W

Metabolic support: NAD+ is essential for efficient metabolism

just my observation

Immunoblotting Protein extracts from adipose tissue mitochondria or cells were prepared using homogenization in radioimmunoprecipitation assay buffer containing protease inhibitors (Sigma-Aldrich)

Avoid placing it in the freezer as the low temperatures can degrade the peptide chains, compromising the effectiveness of your preparation

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