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While the endosome-dependent pathway is acknowledged as a principal mechanism for Exos biogenesis, certain investigations indicate that direct budding from the plasma membrane also contributes to a considerable fraction of Exos formation [47, 66, 67]

For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT

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Individual herbs had varied effects on histamine release, peanut sIgE, and IgG2a levels [37]