Neurons were dispersed by gentle titration through pipettes and they were washed three times with fresh NbActive4 media (BrainBits) and plated onto poly-D-lysine/laminin (BD Bioscience)-coated coverslips, followed by incubation (5% CO 2 in air at 37 C) for 24 h
The c-Myc oncogene directly induces the H19 noncoding RNA by allele-specific binding to potentiate tumorigenesis
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