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At specific time points (0, 4, 8, 24 and 48 hours), 1.0 mL of nanoparticle suspension was centrifuged at 19354 g for 10 minutes (Centurion Scientific Ltd., UK)

The enzymatic deficiency in these individuals causes an increase in -AASA and its cyclic form piperideine-6-carboxylate (P6C)

One observational study involving data from 135 migraine patients indicated treatment with folic acid was more effective than conventional treatment for reducing pain scores in carriers of the MTHFR mutation (Guo 2022)