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The answer lies in the slowed gastric emptying

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Results were gradual but very visible

Following treatment, the medium was removed, cells were washed 2 times with warm PBS, and fixed with 4% formaldehyde for 20 min

According to the corresponding LineweaverBurk plots, the enzyme kinetic parameters, including the maximum reaction rate ( V max ) and MichaelisMenten constant ( K m ), were calculated (Fig

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