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Some users add the upper arm as a fifth site

and Wheeler et al

2b, 3e)

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After 28 days of incubation at 15 C, the enrichment was sub-cultured to remove carry-over contaminants from the initial slurry and 3 mL were stored at 80 C for DNA analysis

subtilis strain is fast growing and can produce riboflavin within 48 h, which makes it preferable for large-scale production (Paracchini et al., 2017)