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Unlike common drugs, peptides do not leave long-lasting metabolites because they break down into amino acids

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Moreover, not all methodologies utilized in these studies are properly validated against the gold standard, i.e., the Ussing chamber, and therefore caution is needed in the interpretation of these permeability data

A minimum of 98% is considered research grade, though 99% is strongly preferred for sensitive assays, cell culture experiments, and in vivo pre-clinical studies where compound purity directly affects reproducibility and result accuracy

doi: 10.3389/fimmu.2022.995582 Received 16 July 2022 Accepted 01 November 2022 Published 16 November 2022 Volume 13 - 2022 Edited by Luiz Eduardo Baggio Savio, Federal University of Rio de Janeiro, Brazil Reviewed by Cassio Almeida-da-Silva, University of the Pacific, United States