The resulting supernatant was used for subsequent assays
According to the UK government, our bodies require 1.5 micrograms (mcg) a day of Vitamin B12, although other governments advise that we actually need more
The association that developed between the response and the independent elements was modeled using the following second-order polynomial equation: In the statistical model, the dependent variable is represented by Y, the independent variables by X i and X j , and the coefficients for the intercept, linear terms, quadratic terms, and interaction terms are denoted by 0 , i , ii , and ij , respectively
Fixed cells were washed twice in PBS containing 10% serum and stained with 50 g ml 1 propidium iodide solution containing 100 g ml 1 RNase A for 1 h at 37 C
Astrocytes are glycolytic
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