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256 Specifically, in normal cells of the central nervous system, p53 is usually expressed in nonneuronal cells
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Kelley WN, Holmes EW, Van der Weyden MB
After being washed with PBS, the cells were permeabilized with 0.1% Triton X-100 for 5 min at room temperature and blocked with 3% bovine serum albumin with glycine for 30 min
