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Proteins were then transferred onto a polyvinylidene difluoride (PVDF) membrane using an iBlot2 PVDF stack (Invitrogen) and blocked with 5% bovine serum albumin in tris-buffered saline with 0.1% Tween-20 (TBST) for 1 hour at room temperature

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Pregnant women need any higher intake of Vitamin B12