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The incubated sample was loaded onto a Superdex 200 Increase SD200 column (Cytiva), equilibrated with 20 mM HEPES pH 8, 200 mM NaCl, 1 mM DTT, for size-exclusion chromatography, and fractions containing all subunits of the SCF FBXO22 -Degrader-NSD2 PWWP1 complex were pooled and concentrated

Vitamins B3 (niacinamide), B5 (pantothenic acid), and B7 (biotin) are particularly relevant

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These findings emphasize the effectiveness of COVID-19 vaccinations in cancer patients, while underscoring the necessity for customized immunization regimens for immunocompromised populations, particularly individuals with hematologic malignancies and those undergoing treatments that compromise B-cell function [327]