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Adding protease inhibitors such as 1mM PMSF to cell lysates does not inhibit binding or elution of polyhistidine-tagged proteins with the HisLink Resin and is highly recommended to prevent degradation of the protein of interest by endogenous proteases

The mechanism is simple and well understood: fibre swells in the digestive tract, slows gastric emptying and prolongs the feeling of fullness

Common sites are the abdomen and thigh

10.1038/s41419-021-03452-x 44 KimM.BaeJ

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Vitamin D promotes the expression of neuroprotective factors, including neurotrophins and antioxidant enzymes, which help prevent neurodegeneration and oxidative stress [141]