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For apoptosis detection, the DAB (SA-HRP) TUNEL Cell Apoptosis Detection Kit (G1507-50T, Servicebio) was used to stain paraffin-embedded sections

Compared to BPC-157, which acts primarily via the nitric oxide and growth hormone receptor pathways, and TB-500, which functions through actin sequestration and VEGF signalling, GHK-Cu operates largely through copper-mediated gene expression modulation and extracellular matrix remodelling

Remember, each person's recovery timeline is unique