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"Running out" of B12 usually reflects falling serum levels rather than total depletion

Curr Drug Targets Inflamm Allergy 2: 216223

On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

Homocysteine is a known trigger of redox stress and unfolded protein response in eukaryotic cells

Thus dosage, pharmacokinetics, long-term safety, off-target effects in humans remain unknown

Incorrect amino acid sequences, bacterial endotoxins, and other impurities can cause reactions attributed incorrectly to BPC-157 itself