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Abstract The spectrophotometric/microplate reader assay method for glutathione (GSH) involves oxidation of GSH by the sulfhydryl reagent 5,5-dithio-bis(2-nitrobenzoic acid) (DTNB) to form the yellow derivative 5-thio-2-nitrobenzoic acid (TNB), measurable at 412 nm

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Its famous claim of being roughly seven million times more potent than BDNF is a cell-culture potency measurement, and two foundational papers behind that body of work were retracted in April 2025 for fabricated data