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Proteins from each treatment were loaded in technical duplicate, resolved by SDS-PAGE on NuPAGE 4 to 12%, Bis-Tris, 1.0 mm polyacrylamide gels (Invitrogen) and transferred to a PVDF membrane using the iBlot 2 Transfer Stacks, PVDF, mini and Gel Transfer Device (Invitrogen)

Nat Immunol (2011) 12(3):25563

meningitidis population is thought to be a primary factor in the adaptive evolution of meningococcus, increasing overall spontaneous mutation rates and the rate of phase variation of contingency genes (Saunders et al., 2000

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About Michael Growing up Michael struggled with his weight from a very early age