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RT-qPCR was performed using an SYBR Premix EX Taq kit according to the manufacturers instructions (Takara, Shiga, Japan)

5.4.2 Laser and light-based therapies Laser and light-based therapies are modalities that are utilizing light energy to treat skin lesions (Pitowska et al., 2022)

Gut-brain axis interactions and gastrointestinal cytoprotection

Updated 2024 Feb 23

the sponsor had no interference on data extracting, analyses and manuscript writing

However, in children with normal neonatal courses or clear withholding behaviors, or in whom trauma is suspected, the rectal examination may be deferred